Temporal expression of three conserved putative microRNAs
in response of Citrus × Limon to Xanthomonas citri subsp. citri
and Xanthomonas fuscans subsp. Aurantifolii

Milad Alizadeh; Hossein Askari; Massod Soltani Najafabadi

doi:10.5114/bta.2017.70803

eISSN: 2353-9461
ISSN: 0860-7796

BioTechnologia

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abstract:

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Temporal expression of three conserved putative microRNAs in response of Citrus × Limon to Xanthomonas citri subsp. citri and Xanthomonas fuscans subsp. Aurantifolii

Milad Alizadeh

¹

,

Hossein Askari

¹

,

Massod Soltani Najafabadi

²

Shahid Beheshti University, Tehran, Iran
Oil Seeds Research Department, Seed and Plant Improvement Institute, Karaj, Iran

BioTechnologia vol. 98(3) C pp. 257-264 C 2017

DOI: https://doi.org/10.5114/bta.2017.70803

Online publish date: 2018/01/02

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Citrus canker is a widespread bacterial disease that severely affects Citrus production. Xanthomonas spp. are the causative agents of the disease, which manifests as necrotic pustule-like lesions on the plant organs including leaves, fruits, and stems. MicroRNAs (miRNAs) are well-established post-transcriptional gene expression regulators that modulate plant responses during many stress conditions. Accordingly, some bacteria-responsive miRNAs have been shown to have regulatory functions in the plant-pathogen interactions. In this study, we successfully assessed the expression patterns of three conserved miRNAs: miR159, miR167, and miR398, in Citrus × Limon (lemon) infiltrated with Xanthomonas strains A and C using stem-loop RT-qPCR. Our results showed that the expressions of miR159 and mir167 have nearly similar patterns upon inoculations, while the expression of miR398 remains constantly up-regulated after an early induction.

keywords:

Temporal expression of three conserved putative microRNAs in response of Citrus × Limon to Xanthomonas citri subsp. citri and Xanthomonas fuscans subsp. Aurantifolii

Milad Alizadeh 1 , Hossein Askari 1 , Massod Soltani Najafabadi 2

lemon, Citrus canker, conserved miRNAs, stem-loop RT-PCR, temporal expression

Milad Alizadeh

¹

,

Hossein Askari

¹

,

Massod Soltani Najafabadi

²