eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
1/2023
vol. 48
 
Share:
Share:
abstract:
Experimental immunology

Prognostic values of regulatory T cells (Tregs) and Treg-related genes in gastric cancer

Liang Zheng
1
,
Luping Lin
1
,
Jintian Song
1
,
Sha Huang
1
,
Lizhu Chen
1
,
Hui Li
1
,
Ning Ma
1
,
Qingyue Chen
1
,
Yigui Chen
1

  1. Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, China
Cent Eur J Immunol 2023; 48 (1): 14-25
Online publish date: 2023/04/20
View full text Get citation
 
PlumX metrics:
Introduction:
This study attempted to investigate the potential of a risk model constructed for regulatory T cells (Tregs) and their related genes in predicting gastric cancer (GC) prognosis.

Material and methods:
We used flow cytometry to detect the content of CD4+CD25+ Tregs. After detecting expression of five Treg-related genes by quantitative real-time polymerase chain reaction (qRT-PCR), Pearson analysis was employed to analyze the correlation between Tregs and related gene expression. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation and transwell assays were used to detect the effects of a disintegrin and metalloproteinase with thrombospondin motifs 12 (ADAMTS12) on cell functions. A prognostic risk model was built after Cox regression analysis. The Kaplan-Meier method was employed to assess how Tregs, 5-gene risk scores and expression of 5 genes were correlated with the survival time.

Results:
A significantly increased content of Tregs was found in GC tissues (p < 0.05). 5 Treg- related genes were significantly up-regulated in GC with a positive correlation with the content of Tregs (p < 0.05). Overexpression of ADAMTS12 significantly enhanced the viability, proliferation, migration and invasion of tumor cells. Kaplan-Meier analysis demonstrated poor overall survival and disease-free survival in the high-risk group. The results of survival analysis of Treg content and related gene expression were consistent with those of Cox analysis.

Conclusions:
The risk model constructed based on five Treg-related genes can enable effective prediction in the prognosis of GC patients.

keywords:

Tregs, feature gene, gastric cancer, prognosis

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.