eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
3/2018
vol. 43
 
Share:
Share:
abstract:
Experimental immunology

Influence of trans-resveratrol on macrophage and lymphocyte activity in rainbow trout (Oncorhynchus mykiss) – in vitro study

Elżbieta Terech-Majewska
,
Andrzej Krzysztof Siwicki
,
Sylwia Hermańska
,
Edyta Kaczorek-Łukowska
,
Joanna Pajdak-Czaus
,
Patrycja Schulz

(Centr Eur J Immunol 2018; 43 (3): 241-247)
Online publish date: 2018/10/30
View full text Get citation
 
PlumX metrics:
The objective of this study was to investigate the effect of trans-resveratrol, a potent antioxidant with anti-inflammatory and chemopreventive properties, naturally occurring in many fruits and plants on lymphocytes proliferation and also on macrophages metabolic and phagocytic activity. The aim of this study was to demonstrate the immunomodulatory effects of the compound on fish immunocompetent cells and determine the type of this interaction (immunosuppression or immunostimulation). Proliferative activity of lymphocytes was studied by MTT assay, and the respiratory burst was evaluated using the respiratory burst activity (RBA) test. Phagocytic killing was tested using the PKA test. The experiment have shown that trans-resveratrol suppressed blood B cells, while there was no significant influence on blood T lymphocytes. However, insignificant stimulatory effect occurred at the lowest concentration. In addition, the compound inhibited proliferation of T and B lymphocytes isolated from the organs. Importantly, trans-resveratrol caused stimulation of blood and organs macrophages phagocytic killing, and also increased the respiratory burst of macrophages isolated from organ. These results suggest a potential use of trans-resveratrol as an immunomodulator of innate immunity in fish. This is particularly important, as this kind of resistance plays leading role in protecting the body against infection. In comparison, adaptive immunity is slower and also much less precise.
keywords:

trans-resveratrol, in vitro studies, immunomodulators, cellular immunity

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.