eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
2/2024
vol. 49
 
Share:
Share:
Report

Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom

Ivana Lukic
1
,
Veljko Blagojevic
1
,
Rajna Minic
1
,
Sasa Ivanovic
2
,
Suncica Borozan
3
,
Vitomir Cupic
2
,
Irena Zivkovic
1

  1. Institute of Virology, Vaccines and Sera “Torlak”, Belgrade, Serbia
  2. Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, University of Belgrade, Belgrade, Serbia
  3. Faculty of Veterinary Medicine, Department of Chemistry, University of Belgrade, Belgrade, Serbia
Cent Eur J Immunol 2024; 49 (2): 94-104
Online publish date: 2024/08/26
Article file
Get citation
 
PlumX metrics:
 
1. World Health Organization (2023): Available from: https://www.who.int/news-room/fact-sheets/detail/snakebite-envenoming (accesed on 3.10.2024).
2. Chippaux JP (2012): Epidemiology of snakebites in Europe: a systematic review of the literature. Toxicon 59: 86-99.
3. Ratanabanangkoon K (2023): Polyvalent snake antivenoms: production strategy and their therapeutic benefits. Toxins (Basel) 15: 517.
4. Council of Europe (2014): European Pharmacopoeia 2014. Available from: https://pheur.edqm.eu/home.
5. Georgieva D, Risch M, Kardas A, et al. (2008): Comparative analysis of the venom proteomes of Vipera ammodytes ammodytes and Vipera ammodytes meridionalis. J Proteome Res 7: 866-886.
6. Leonardi A, Sajevic T, Pungerčar J, et al. (2019): Comprehensive study of the proteome and transcriptome of the venom of the most venomous european viper: discovery of a new subclass of ancestral snake venom metalloproteinase precursor-derived proteins. J Proteome Res 8: 2287-2309.
7. Kurtovic T, Leonardi A, Lang Balija M, et al. (2012): The standard mouse assay of anti-venom quality does not measure antibodies neutralizing the hemorrhagic activity of Vipera ammodytes venom. Toxicon 59: 709-717.
8. Segeritz C, Vallie L: Cell Culture: Growing Cells as Model Systems In Vitro. In: Basic Science Methods for Clinical Researchers, Elsevier 2017; 151-172.
9. Pamies D, Hartung T (2017): 21st century cell culture for 21st century toxicology. Chem Res Toxicol 30: 43-52.
10. Geraghty RJ, Capes-Davis A, Davis JM, et al (2014): Guidelines for the use of cell lines in biomedical research. Br J Cancer 111: 1021-1046.
11. von Keutz E. Toxicity Testing In Vitro: Regulatory Aspects. In: Reichl FX, Schwenk M (eds.) Regulatory Toxicology. Springer 2021; 139-148.
12. Mosmann T (1983): Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 16: 55-63.
13. Lü L, Zhang L, Wai MSM, et al. (2012): Exocytosis of MTT formazan could exacerbate cell injury. Toxicol in Vitro 26: 636-644.
14. Milovanovic V, Minic R, Ivanovic S, et al. (2021): MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation. Toxicon 192: 57-65.
15. Feoktistova M, Geserick P, Leverkus M (2016): Crystal violet assay for determining viability of cultured cells. Cold Spring Harb Protoc 2016: pdb.prot087379.
16. Strober W (2015): Trypan blue exclusion test of cell viability. Curr Protoc Immunol 111: A3.B.1-A3.B.3.
17. Medicines and Medical Devices Agency of Serbia (ALIMS): Viekvin® patient information leaflet, Marketing Authorisation number and date: 515-01-01036-21-001, 20 September 2021.
18. Lonati D, Giampreti A, Vecchio S, et al. (2016): Antivenom availability and clinical response to treatment in viper envenomation in Italy: 3 years’ preliminary experience. 36th International Congress of the European Association of Poisons Centres and Clinical Toxicologists (EAPCCT) 24-27 May, 2016, Madrid, Spain. Clin Toxicol 54: 344-519.
19. Milovanovic V, Dimitrijevic L, Petrusic V, et al. (2018): Application of the 3R concept in the production of European antivenom on horses – multisite, low volumes immunization protocol and ELISA. Acta Veterinaria-Beograd 68: 401-419.
20. World Health Organization (2017): WHO Expert Committee on Biological Standardization Sixty-seventh report, WHO TRS No 1004, 2017. Annex 5: Guidelines for the production, control and regulation of snake antivenom immunoglobulins; 197-388.
21. Ammerman NC, Beier-Sexton M, Azad AF (2008): Growth and maintenance of Vero cell lines. Curr Protoc Microbiol Appendix 4: Appendix 4E.
22. Kasibhatla S, Amarante-Mendes GP, Finucane D, et al. (2006): Acridine orange/ethidium bromide (AO/EB) staining to detect apoptosis. CSH Protoc 2006: pdb.prot4493.
23. Bundscherer A, Malsy M, Lange R, et al. (2013): Cell harvesting method influences results of apoptosis analysis by annexin V staining. Anticancer Res 33: 3201-3204.
24. Merten OW (2015): Advances in cell culture: anchorage dependence. Philos Trans R Soc Lond B Biol Sci 370: 20140040.
25. Khalili A, Ahmad M (2015): A review of cell adhesion studies for biomedical and biological applications. Int J Mol Sci 16: 18149-18184.
26. Gasanov SE, Dagda RK, Rael ED (2014): Snake Venom Cytotoxins, Phospholipase A2s, and Zn2+-dependent Metalloproteinases: Mechanisms of Action and Pharmacological Relevance. J Clin Toxicol 4: 1000181.
27. Halassy B, Brgles M, Habjanec L, et al (2011): Intraspecies variability in Vipera ammodytes ammodytes venom related to its toxicity and immunogenic potential. Comp Biochem Physiol C Toxicol Pharmacol 153: 223-230.
28. Araki S, Ishida T, Yamamoto T, et al. (1993): Induction of apoptosis by hemorrhagic snake venom in vascular endothelial cells. Biochem Biophys Res Commun 190: 148-153.
29. Nalbantsoy A, Karabay-Yavasoglu NU, Sayim F, et al. (2012): Determination of in vivo toxicity and in vitro cytotoxicity of venom from the cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production. J Venom Anim Toxins Incl Trop Dis 18: 208-216.
30. Nowak-Terpiłowska A, Śledziński P, Zeyland J (2021): Impact of cell harvesting methods on detection of cell surface proteins and apoptotic markers. Braz J Med Biol Res 54: e10197.
31. Al-Asmari AK, Riyasdeen A, Al-Shahrani MH, et al. (2016): Snake venom causes apoptosis by increasing the reactive oxygen species in colorectal and breast cancer cell lines. Onco Targets Ther 9: 6485-6498.
32. Wlodkowic D, Skommer J, Darzynkiewicz Z (2012): Cytometry of apoptosis. Historical perspective and new advances. Exp Oncol 34: 255-262.
33. Renvoize C, Biola A, Pallardy M, et al. (1998): Apoptosis: identification of dying cells. Cell Biol Toxicol 14: 111-120.
Copyright: © 2024 Polish Society of Experimental and Clinical Immunology This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.