eISSN: 2391-6052
ISSN: 2353-3854
Alergologia Polska - Polish Journal of Allergology
Current issue Archive Manuscripts accepted About the journal Supplements Special issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
2/2024
vol. 11
 
Share:
Share:
Review paper

Biological treatment options for eosinophilic asthma, with consideration of markers of eosinophilic inflammation as predictors of treatment efficacy

Aleksandra Niemiec
1
,
Olga Branicka
1
,
Barbara Rymarczyk
1
,
Radosław Gawlik
1

  1. Department of Internal Diseases, Allergology and Clinical Immunology, Medical University of Silesia, Katowice, Poland
Alergologia Polska – Polish Journal of Allergology 2024; 11, 2: 163–172
Online publish date: 2024/02/17
Article file
Get citation
 
PlumX metrics:
 

INTRODUCTION

Asthma is a chronic inflammatory disease of the airways characterized by reversible airflow obstruction and easily triggered bronchospasms which affects more than 300 million people worldwide [1]. Symptoms include cough, particularly at night or early in the morning, wheezing and shortness of breath. The episodes are usually associated with allergen contact, irritants or air pollution and they can be suppressed spontaneously or with treatment. Factors e.g. viral infections, mold, cigarette smoke, exercise, cold air, some medications (aspirin, β-blockers) and, obviously, allergens may trigger exacerbations. Avoiding triggers mentioned above and effective pharmacotherapy may ensure good quality of life and proper asthma control [2].

It was thought for a long time that asthma is a homogenous disease. Nowadays, it is known that there are many mechanisms that could cause asthmatic symptoms. Several phenotypes and endotypes of asthma have been described. Initially, asthma phenotypes were defined by combinations of clinical features. In 2012, Wenzel sought to link biology to symptoms by describing six distinct kinds of asthma: early-onset allergic, late-onset eosinophilic, exercise-induced, obesity-related, neutrophilic, and paucigranulocytic asthma [3]. The term endotype refers to a subtype of a disease defined functionally and pathologically by a molecular mechanism or by treatment response according to GINA. The present-day classification divided to two basic groups T2-high and T2-low driven disease relies on the level of bronchial eosinophilia [4].

Type 2 immunity is induced by Th2 (T helper lymphocytes) and is associated with an increased production of the cytokines related with Th2 including interleukin-4 (IL-4), IL-5, IL-9, and IL-13. Molecules with protease activity, e.g. allergens, can break cell tight junction proteins and get access to submucosal dendritic cells (DC). Damaged epithelial cells release cytokines such as TSLP, IL-25, and IL-33, which activate innate lymphoid cells (ILC) and DC. Mature DC move to nearby lymph nodes and they use MHC class II molecules to deliver allergen peptides to naïve T cells. Type 2 cytokines are produced by Th2 as well as ILC2 cells. IL-4 and IL-13 stimulate lymphocytes B to class-switch to IgE. In order to sensitize mast cells (MC), IgE binds to receptor FcRI (epsilon) on their surface. Mast cell-associated mediators such as tryptase, histamine, leukotrienes, prostaglandins, are released, causing smooth muscle contraction and hyperplasia of goblet cells. Then Th2 cytokines are produced and released [5, 6]. They play an important role in the inflammation process, e.g. IL-9 induces eosinophilic inflammation, mucus hypersecretion and hyperresponsiveness of the airway, whereas IL-5 play a crucial role in activation, recruitment and survival of eosinophils. On the other hand, T-regulatory (Treg) cells can decrease type 2 response by releasing immunoregulatory cytokines such as IL-10 and transforming growth factor-β (TGF-β) [7, 8] (Table 1).

TABLE 1

Comparison of inflammatory phenotypes of asthma [8]

FeatureType 2 asthmaNon-type 2 asthma
Age of onsetChildhood, young ageAdulthood, later onset
SymptomsMay be signi-ficantMay be signi-ficant
Exacerbation rateHigherLower
ObstructionMoreLess
SABA responseBetterWorse
Exhaled NONormal to elevatedLow to normal
Airway eosinophiliaPresentAbsent
Allergic sensitizationPresentAbsent
Response to corticosteroidsBetterWorse
ObesityMay be presentFrequent

ROLE OF EOSINOPHILS IN ASTHMA

Eosinophils are mature, definitely differentiated, acidophilic granulocytes that play a significant role in innate host defense against pathogens, especially parasites. Eosinophils have ability to damage pathogenic and also host cells through the release of toxic granule proteins and reactive oxygen species (ROS) [9].

Activated eosinophils contribute to inflammation of the airway and cause destruction of the bronchial mucosa by releasing multiple chemokines, cytokines, growth factors and lipid mediators. Increasing scientific evidence indicates that eosinophils play a significant role as effector cells involved in airway remodeling, a phenomenon characterized by subepithelial fibrosis, with fibroblast and myofibroblast accumulation beneath the subepithelial basement membrane, increased TGF-β1 expression, and excessive extracellular matrix (ECM) protein and metalloproteinase (MMP) deposition in the stroma underlying the bronchial epithelium [10]. Other changes of the airway structure observed in asthma include an increase in smooth muscle mass, goblet cell hyperplasia, and new blood vessel formation. The end result is increased thickness of the bronchial wall, leading to a reduction in the airway caliber, an exaggerated airway narrowing, and a progressive decline of respiratory function [11, 12]. Through effector mechanisms mentioned above, eosinophils have a great impact on tissue specific function [10]. Major basic protein (MBP), also known as proteoglycan 2, is an eosinophil granule protein. MBP’s biological activities are mostly connected to its cytotoxic properties. It changes the charge of surface membranes, which results in disrupted permeability, disruption and harm to cell membranes. MBP plays a crucial role in eosinophil-mediated host defense against helminth infection. However, elevated levels of MBP have been also observed at eosinophil-rich sites of inflammation e.g. in bronchial epithelium in asthmatics. MBP activates histamine release by basophils and mast cells [13]. Eosinophil cationic protein (ECP) is another eosinophil granule protein. ECP plays an important role in eosinophil-related disorders, particularly in asthma. According to scientific studies, eosinophil overexpression corresponds with asthma severity and exacerbation frequency. In asthmatics, increased amounts of eosinophils are present in blood, in sputum, and bronchoalveolar fluid [14, 15].

Eosinophilic asthma typically presents in adulthood (40–50 years of age) and is characterized by T2-high eosinophilic inflammation of the respiratory tract [16]. Exacerbations are frequent and patients are often dependent on oral corticosteroids. Chronic rhinosinusitis and nasal polyposis could coexist with eosinophilic inflammation of the airways [17].

EOSINOPHILS’ HETEROGENEITY

Eosinophils’ primary role in both health and sickness is to control local immunity and remodeling/repair [9]. Eosinophils play a role in host defense against various pathogens, tissue homeostasis and exhibit immunomodulatory activities. Eosinophils promote allergic inflammation through the release of pro-inflammatory mediators, proteins. The eosinophils’ twin responsibilities in creating and suppressing inflammation imply that functional subtypes of eosinophils may exist. ‘Resident eosinophils’ (rEos) are present in healthy tissues in the absence of inflammation, in various locations e.g. in the airways, the uterus, the thymus and adipose tissue. However, they are most prevalent in the gastrointestinal tract. Eosinophils are found in almost every part of the gastrointestinal system, excluding the esophagus. Eosinophils in the intestine contribute to mucosal immunological homeostasis. For instance, the absence of eosinophils is related with decreased secretory IgA production at the intestinal mucosa, changes in the intestinal microbiome, and dysregulated mucosal barrier integrity [18]. Resident eosinophils are suggested to help maintain tissue homeostasis [19, 20]. The other type of eosinophils are inducible (iEos), which are IL-5 dependent cells and are associated with inflammatory conditions e.g. asthma, eosinophilic esophagitis (EoE). They are typically triggered by an allergic reaction to dietary or environmental allergens via eosinophil-derived mediators (including eotaxin-3, IL-5, IL-13, TGF-β, and periostin) [21].

Mesnil et al. conducted a mouse study to evaluate the role of rEo in the lungs. Firstly, it was proven that rEo and iEo differ from each other. They have dissimilar gene profile and expression of biomarkers [22]. iEos expressed a high level of various proinflammatory genes, including Slc3a2, Tlr4, C3ar1, Il13ra1, and Il6, consistent with their established function as proinflammatory effector cells. While, rEos expressed several genes involved in the negative regulation of immunological responses and tissue homeostasis, including Anxa1, Runx3, Nedd4, Ldlr and Serpinb1a [23, 24]. Flow cytometry was used to examine the expression of numerous surface molecules on rEos and iEos, e.g. Singlec-F, CD62L, C101L and CD125. CD 62L, known also as L-selectin, is a cell adhesion molecule found on leukocytes including eosinophils. CD62L was shown to be expressed on the surface of rEos but not on the surface of iEos. On the other hand, rEos displayed modest amounts of CD101 (immunoglobulin superfamily, member 2), while CD101 was strongly expressed on the surface of iEos. CD62L and CD101 expression distinguishes between mouse lung rEos and iEos. Considering the above, CD101 could be a useful marker to discriminate between rEos (CD101lo) and iEos (CD101hi) [22].

MARKERS OF EOSINOPHILIC INFLAMMATION

There are several markers used for the determination and monitoring of eosinophil-associated diseases. Concerning respiratory diseases we commonly use the following markers: the number of circulating eosinophils, organ-specific eosinophil levels, fractional exhaled nitric oxide (FeNO) [25].Published studies have revealed eosinophil count as a promising marker for Th2-high asthma. We should remember that circulating eosinophils are present in the blood for around 8–18 h, in contrast to their prolonged life span of up to 14 days in tissue [26]. Eosinophils undergo migration from the bloodstream to the tissues. Typically, once eosinophils migrate into the tissues, the majority of them do not circulate again [26]. The number of circulating eosinophils in human peripheral blood ranges between 50 and 500/µl under normal conditions. Additionally, it is worth noting that blood eosinophils vary by around 20% during the day. The fluctuation in eosinophil levels frequently refers to the suppressive impact of cortisol hormones. The levels of cortisol are at their peak in the morning and decrease throughout the night, resulting in lower eosinophil counts in the morning and higher numbers at night [27].

FeNO concentration of 25 parts per billion (ppb) in adults indicates the presence of eosinophilic airway inflammation and serves as an indicator of the probability of responding to corticosteroid treatment [28]. Regarding spirometric measurements, there was a noticeable inverse association between the FeNO level and the FEV1% and a slightly positive relation between the FeNO level and bronchodilator reversibility [28]. Furthermore, a high FeNO level is regarded as a risk factor for poorly managed asthma and subsequent episodes of exacerbations [29].

Periostin is a matricellular protein, which plays a significant role in the remodeling and type-2 airway inflammation seen in asthma. Yuyama et al. originally showed that the expression of the POSTN gene, which encodes periostin, is upregulated by two essential type-2 cytokines in asthma: IL-4 and IL-13 [30]. Its production in airway epithelial cells is associated with the thickness of the airway basement membrane. Periostin may be reliably identified in blood, suggesting airway type-2 inflammation and remodeling [31]. Additionally, serum periostin was found to be a reliable indicator of the efficacy of lebrikizumab, an anti-IL-13 antibody, in treating patients with unstable asthma, as demonstrated in a phase 2b research. The group with elevated serum periostin levels exhibited a more significant improvement in FEV1 in response to lebrikizumab compared to the placebo group. However, this effect was not detected in the group with low serum periostin levels [32].

In fact, blood eosinophil counts, FeNO levels and serum periostin levels may represent markers of type-2 inflammation [33].

Moreover, density of eosinophils may play a significant role in asthma [34]. Two different cell types of eosinophiles have been discovered depending on cell density: normal and hypodense (< 1.085 g/l). The latter seem to be an activated phenotype. In healthy individuals peripheral blood show a low number of normodense and hypodense eosinophils, in contrast with the high amount of hypodense cells in patients suffering from allergies [35]. Higher cytotoxic eosinophil abilities were observed in the case of the hypodense cell population [36]. Lower density cells are characteristic of an increased number of receptors for complement and immunoglobulins, as well as an increased response to chemotaxis and intensified metabolic activity. Kuo et al. observed that the percentage of hypodense eosinophils is strongly related to severity of asthma. Patients diagnosed with mild asthma have a lower percentage of hypodense eosinophils in the blood than those with moderate asthma [37]. Furthermore, the distribution profiles of eosinophils changes to a prevalence of normodense cells after corticosteroid treatment, suggesting that corticosteroid drugs influence the eosinophil activation rather than simple decrease of the eosinophilic cell number [37, 38]. Experiments performed so far indicate that the clinical efficacy of corticosteroids might be related to inhibition of the eosinophil conversion into hypodense subpopulations [37].

AVAILABLE TREATMENT

Eosinophil-targeted biologics are divided according to their mechanisms of eosinophil reduction: IL-5 depletion (mepolizumab and reslizumab), antibody-dependent cytotoxicity (benralizumab) and interference with eosinophil transport to tissues (dupilumab). Mepolizumab binds soluble IL-5, preventing it from attaching to the IL-5 receptor on eosinophils and thereby suppressing eosinophil growth and activation [39]. Benralizumab is a recombinant humanized monoclonal antibody which possesses the particular feature of binding to a conformationally distinct epitope within domain 1 of the IL-5R-chain with high affinity. The absence of fucose (afucosylation) rockets its affinity for CD16a and triggers antibody-dependent cell-mediated cytotoxicity by natural killer (NK) cells [40]. In turn, dupilumab is a monoclonal antibody which targets the IL-4 receptor a chain (IL-4Rα). There exist two types of receptors: type I receptor, composed of IL-4Rα/γc heterodimers, which exclusively binds IL-4, and type II receptor, consisting of IL-4Rα/IL-13Rα1, capable of binding both IL-4 and IL-13. Activation of these receptors initiates a cascade of signaling events, prominently involving JAK kinases (Janus kinase), such as JAK1/JAK3 (for type I receptors) and JAK1/Tyk2 (for type II receptors). Activated JAK kinases trigger further phosphorylation of specific tyrosine residues within the cytoplasmic domain of IL-4Rα, leading to the activation of various signaling pathways, including STAT6, SHC/MAPK, IRS/PI3K/mTORC2/AKT and Shp-1. The IL-4/IL-13/IL-4R axis stimulates the differentiation of T helper cells type 2 (TH2), which mediates the pro-allergic adaptive immune response [41]. Dupilumab reduces type 2 inflammation indicators such as total IgE, periostin and plasma eotaxin-3. In 52-week clinical studies there was observed reduction in median percentage change in serum total IgE from −70.0% to −76.7%. In turn, the median percentage change in plasma eotaxin-3 in dupilumab-treated patients with asthma over placebo was –38.24% versus –0.16%. In a subset of patients with asthma and chronic rhinosinusitis with nasal polips treated with dupilumab, temporary elevations in blood eosinophils were observed, followed by a return to near-baseline levels by the end of treatment [42].

Omalizumab is a humanized monoclonal anti-IgE antibody. It is dedicated for severe allergic asthma treatment, but also for other atopic disease – chronic spontaneous urticaria. Omalizumab inhibits free serum IgE binding to the FcεRI receptor on the surface of mast cells and basophils [43]. Additionally, omalizumab has also been shown to diminish the expression of FcεRI on the surface of circulating mast cells and basophils, resulting in a reduction in the release of allergic mediators [44] (Table 2).

TABLE 2

Biological drugs available in Poland used in the treatment of severe asthma

Biological drugBrand nameMechanism of actionDose [mg]Frequency of administration [weeks]Route of administration
OmalizumabXolairAnti-IgE75–6004Subcutaneous
MepolizumabNucalaAnti-IL 51004Subcutaneous
ReslizumabCinqaeroAnti-IL-53 per 1 kg of bodyweight4Intravenous
BenralizumabFasenraAnti-IL5 R304 (first 3 times), then 8Subcutaneous
DupilumabDupixentAnti-IL4 R600 mg (1. dose); 300 mg2Subcutaneous

Additionally, there is a novel monoclonal antibody – tezepelumab, acting upstream, which is not yet available in Poland. Tezepelumab selectively inhibits the binding of thymic stromal lymphopoietin (TSLP) to its heterodimeric receptor. TSLP is secreted by the airway epithelium in response to many stimuli, including allergens, viruses and air pollutants, which initiates an inflammatory cascade [45]. Excessive production of TSLP can cause abnormal inflammation, which can result in worsening of asthma symptoms, hyperresponsiveness and structural changes in the airways [46]. The beneficial effects and safety of tezepelumab in individuals with severe, uncontrolled asthma were assessed in the randomized, placebo-controlled phase 2b PATHWAY and phase 3 NAVIGATOR trials. Tezepelumab treatment resulted in a decrease in the risk of asthma exacerbations per year when compared to a placebo in participants from both trials. This effect was observed in patients with either high or low baseline blood eosinophil count or FeNO levels, and was independent of the allergy status. Tezepelumab has shown enhancements in pulmonary function, disease control and quality of life. [47, 48].

COMPARISON OF ANTI-EOSINOPHIL BIOLOGICAL DRUGS

Generally, there are three biologics which are used for severe eosinophilic asthma treatment – mepolizumab, benralizumab and dupilumab. Despite their demonstrated efficacy, many questions remain unsolved, especially regarding the comparative effectiveness of the drugs. Clinical trials that have been performed were placebo controlled rather than providing head-to-head comparisons [49].

Akenroye et al. performed a meta-analysis on the efficacy and safety of mepolizumab, benralizumab and dupilumab in treatment of eosinophilic asthma. To account for clinical heterogeneity, as the eosinophil count is determinant of the efficacy of these treatments, researchers concentrated on groups of patients with eosinophil ranges of ≥ 300 cells/ml and 150–299 cells/ml. In the group of patients with eosinophils ≥ 300 cells/ml, all three biological drugs were substantially more effective than placebo in reducing exacerbations. In case of dupilumab (RR = 0.32; 95% CI) reduction of exacerbations was the most noticeable comparing to the other drugs: mepolizumab (RR = 0.37; 95% CI) and benralizumab (RR = 0.49; 95% CI). Improvement of spirometric parameter FEV1 was also the most explicit in the dupilumab group. Mean difference with dupilumab administration was 230 ml; benralizumab 150 ml and mepolizumab 150 ml. The best asthma control assessed by ACQ test was observed in the mepolizumab group. In patients with eosinophils 150–299 cells/ml, benralizumab (RR = 0.62; 95% CI) and dupilumab (RR = 0.60; 95% CI) were associated with lower exacerbation rates. The effect of both biologics was similar and greater than in the mepolizumab group. Improvement of FEV1 was comparable in mepolizumab and benralizumab groups, slightly better than in the dupilumab group. Mepolizumab caused the most serious side effects in both study subgroups [39]. The conclusion of the meta-analysis was that mepolizumab, dupilumab, and benralizumab are characterized by similar efficacy and safety in patients with severe eosinophilic asthma [4953].

REASONS OF TREATMENT INEFFECTIVENESS

In some patients, despite daily use of oral corticosteroids or management with biologics, persistent airway eosinophilia is observed. Emerging theories suggest the existence of localized mechanisms that are resistant to maintenance corticosteroids, delaying inflammation resolution and causing recurrent exacerbations with distinctive clusters of free eosinophil granules (FEGs) in sputum. The elevated concentration of FEGs in the airways shows a robust correlation with the level of sputum eosinophil peroxidase (EPX) released during luminal eosinophil degranulation, signifying an “active” disease [54]. Mukherjee et al. observed the presence of pathogenic autoantibodies in the sputum of patients with persistent eosinophilia and severe asthma. Sputum autoantibodies to EPX (anti-EPX) and various antinuclear antigens, which are not detectable in circulation, imply that a polyclonal autoimmune process specific to the airways has occurred in these patients. The presence of anti-EPX shows a high correlation with the severity of asthma. Moreover, patients with prednisone-dependent eosinophilic asthma showed elevated levels of anti-EPX IgG [55].

Although patients with severe eosinophilic asthma have been properly qualified to biological therapy, they may not achieve expected asthma control and clinical improvement. Treatment with biotherapeutics reduce eosinophil count in the blood, but eosinophils may still be present in the airways [56]. Cytokines/chemokines generated by a variety of cells, including Th2 cells, play a key role in regulating transvascular eosinophil migration. The interaction of eosinophils with endothelial cells via the a4 integrin/vascular cell adhesion molecule (VCAM)-1 pathway appears to be a significant step in selective eosinophil recruitment, according to accumulating data. Blood eosinophils spontaneously attach to VCAM-1 in response to the Th2 cytokines IL-4 and IL-13. The interaction of eosinophils with VCAM-1 increases granule protein release from eosinophils and may thus represent an initial step in the activation of these cells. Even in the absence of IL-5, eosinophilic airway inflammation may be maintained by the Th2 network, which includes a cascade of VCAM-1/CC chemokines/GM-CSF, may sustain eosinophilic infiltration and activation [57, 58].

Eosinophils from asthmatic patients have many phenotypic alterations, particularly in adhesive properties. Barthel et al. observed that human eosinophils recruited to the airway in response to segmental antigen exposure display an allosterically active version of αMβ2, which is associated with greater αMβ2-mediated adherence to various ligands and increased podosome formation. Integrins, including αMβ2 (CD11b/18), α4β1 (CD49d/29), αLβ2 (CD11a/18), are thought to mediate eosinophils rolling and arrest on endothelium, migration through endothelium and the basement membrane in response to chemotactic cues, and bronchial epithelial crossing into the airway lumen [59]. Experiments in humans using anti-IL-5 antibodies show that eosinophils play a role in the processes that lead to the deposition of certain matrix proteins within the reticular basement membrane [60]. In asthmatics who were given three infusions of mepolizumab there was about a 90% reduction in blood and bronchial lavage eosinophils but only 55% of eosinophils in bronchial mucosal were diminished [61].

In fact, the number of eosinophils in the blood does not appear to be an adequate marker of the effectiveness of therapy. During dupilumab treatment, transient eosinophilia was observed. Also single cases of eosinophilic granulomatosis with polyangiitis have been described. Despite transiently elevated eosinophil counts, most patients show improvement in asthma control, exacerbation frequency and spirometric parameters [62].

POSSIBLE NEW ANTI-EOSINOPHIL BIOLOGICAL DRUGS

New possible targeted anti-eosinophil drugs may block eosinophil receptors of potent eosinophil chemokines. CCR3 is the related receptor for major human eosinophil chemoattractant, expressed by eosinophils and crucial for their recruitment to the lung tissue through its binding to eotaxin [63]. Eosinophils move through the sinusoidal endothelium in the bone marrow and are released into the peripheral circulation under the effect of IL-5 and eotaxin [64]. The role of eotaxin was investigated in animal models. Aerosol administration of eotaxin to guinea pigs causes an influx of eosinophils into the airways. In turn, deletion of the eotaxin gene in mice reduces the early recruitment of eosinophils after allergen exposure [65].

Another possible future therapy could be anti-IL-33 biologics. In asthmatics, levels of IL-33 have been observed to be significantly higher in the peripheral blood, and IL-33 levels were negatively correlated to FEV1 and positively linked to clinical asthma severity [66, 67].

Sialic-acid-binding immunoglobulin-like lectin (Siglec)-8 is also being studied as a therapeutic target for the treatment of allergy and inflammatory diseases. It is a cell-surface inhibitory receptor, which is selectively expressed on blood and tissue eosinophils and mast cells, weakly on basophils [68]. Higher levels of soluble Siglec-8 were reported in the serum of severe asthmatics in comparison to healthy controls. Anti-Siglec-8 monoclonal antibodies have previously been demonstrated to directly cause apoptosis in isolated human eosinophils. A monoclonal antibody to Siglec-8 (lirentelimab) appears to induce apoptosis of cytokine-primed eosinophils via antibody-dependent cellular cytotoxicity [67, 69]. Youngblood et al. conducted an experiment to evaluate the effect of lirentelimab on tissue eosinophils. By using flow cytometry, eosinophils were phenotyped in order to compare the expression of surface markers in blood and tissue eosinophils. Two cell-surface markers, CD62L and IL-5 receptor, were shown to be strongly expressed on peripheral blood eosinophils but considerably downregulated on human lung tissue eosinophils. In turn, the expression of Siglec-8 on tissue and blood eosinophils was similar. Lirentelimab can effectively reduce both tissue and blood eosinophils [69].

POTENTIAL NOVEL NON-BIOLOGICAL ANTI-EOSINOPHIL DRUGS

The Janus kinase family is involved in the transduction of cytokine-mediated signals by interacting with and activating STAT (signal transducer and activator of transcription) proteins. Dysregulation of the JAK/STAT pathway is known to be involved in the pathogenesis of allergic inflammation, for instance in asthma. In eosinophils, IL-5 signals through the JAK2-STAT1/STAT5 and MAP kinase pathways control the expression of genes essential for cell survival and proliferation. Furthermore, through JAK-STAT, IL-31 has the ability to inhibit eosinophil apoptosis and promote the release of pro-inflammatory cytokines and chemokines [70]. Luschnig et al. investigated the efficacy of the oral JAK inhibitors: baricitinib (JAK1/2) and tofacitinib (JAK3) in reducing eosinophil effector function in vitro and in vivo in an animal model of allergic eosinophilic lung inflammation. It was shown that baricitinib inhibits eosinophil effector function more effectively than tofacitinib. Thus, targeting the JAK1/2 pathway seems to be a better therapeutic option for eosinophilic inflammation [71].

Additionally, Vernet et al. performed a cohort study that was based on gene mapping in asthmatic patients, their first-degree relatives and healthy controls. A couple of genes were evaluated e.g. RNASE2 and RNASE3, which encode eosinophil cationic protein (ECP) and eosinophil-derived neurotoxin (EDN), and also 4 other genes (JAK1) that are responsible for ECP and EDN levels [72]. ECP and EDN are eosinophil proteins which contribute to epithelium damages and airway remodeling. They have been associated with asthma exacerbations and severity of the disease. Higher levels of ECP and EDN have been observed in asthmatic patients than non-asthmatic individuals. Regarding the above mentioned, drugs inhibiting JAK1 have been identified as a potential therapeutic target for eosinophilic asthma [72, 73].

Dexpramipexole is an orally available synthetic aminobenzothiazole, which considerably reduces blood and tissue eosinophils with a good safety profile [74]. Siddiqui et al. performed a randomized controlled trial which assessed the safety and effectiveness of dexpramipexole in decreasing blood and airway eosinophilia in patients with asthma and blood eosinophil count greater than or equal to 300/ml. Available evidence indicates that dexpramipexole lowers eosinophils by preventing their maturation. This means that the substance has no effect on mature cells. Eosinophil count reduction was seen starting at weeks 4 to 6. Nasal eosinophil peroxidase (EPX) is correlated to sputum eosinophil count in patients with severe asthma. An exploratory end point in the study was the nasal EPX week-12 ratio to baseline. The group which was administered 150-mg dexpramipexole twice a day had the highest decrease in nasal EPX. Additionally, nasal EPX ratio to baseline at week 12, was highly correlated with blood eosinophil count reduction [75].

DISCUSSION

Eosinophils belong to a group of white blood cells, granulocytes, and are widely known for their potential to eliminate parasitic infections and their role in type 2 inflammation. Eosinophils produce factors of the type-2 pathway such as interleukin IL-4, IL-5, IL-13 and IL-25. This effect is responsible for developing inflammation and allergic reactions, observed e.g. in the airways of asthmatics [76].

IL-5 plays an important role in eosinophil maturation. It controls the development of eosinophils in the bone marrow, while they differentiate from myeloid precursor cells to the mature form. Humanized anti-IL-5 antibodies (mepolizumab and reslizumab) and also antibody against IL5-R (benralizumab) critically lower eosinophil levels in the blood by preventing eosinophil maturation in the bone marrow [77]. According to Moran et al. study, benralizumab caused an eosinophil depletion that was rapid and nearly complete, and its commencement of action was quite comparable to that of oral prednisolone [78]. The blood eosinophil count was considerably reduced after benralizumab was compared to mepolizumab 30 days following the first injection [78, 79]. However, mepolizumab and benralizumab have demonstrated comparable efficacy and safety in the long-term treatment of severe eosinophilic asthma, suggesting that the difference in onset and efficacy is not relevant in the chronic use of these drugs. Considering that benralizumab has a faster onset of action, it has the potential to be used as an alternate non-corticosteroid therapy for acute asthma exacerbations [78].

Several scientific studies attempted to clarify the response to anti-eosinophil treatments. These studies noted alterations in the expression of certain factors following drug administration. Specifically, it was observed that mepolizumab led to a decrease in the activation of peripheral blood eosinophils through β2 integrin (CD 18), while β1 integrin (CD 29) remained unaffected. The varying outcomes of anti-IL 5 therapy might stem from its selective inhibition of one aspect of eosinophil activation, as opposed to others [80].

Control of eosinophil apoptosis has become a therapeutic strategy for treating allergic diseases and other eosinophil-associated disorders [26]. However, in some cases peripheral blood eosinophils depletion does not ensure expected clinical improvement. Furthermore, dupilumab, monoclonal antibody anti-IL 4 R/anti-IL 13 approved for eosinophilic asthma treatment, may cause transient increase of blood eosinophils. Given this information, it appears that the blood eosinophil count may not be a reliable marker for assessing therapy effectiveness [81].

However, in a routine diagnostic process or qualification to the biotherapies only the total amount of eosinophils in peripheral blood is assessed [82]. Density of the cells or other makers of eosinophilic inflammation are not evaluated. There may be a relation between efficacy of biological treatment and some markers of eosinophilic inflammation. Further studies have to be performed in this field.

CONCLUSIONS

Severe asthma is sometimes still difficult to determine appropriate treatment due to significant clinical heterogeneity. Eosinophils play a significant role in the pathogenesis of asthma. Markers of eosinophilic inflammation can be helpful in the determination of the asthma endotype, qualification for biological treatment and also in its monitoring.

However, availability of different biological drugs and assumedly proper qualification to the drug program do not always bring the success. Some patients still do not achieve expected improvement of the clinical state. The causes of side effects and lack of improvement after biological treatment are still unknown, and further observations and scientific studies are needed.

FUNDING

No external funding.

ETHICAL APPROVAL

Not applicable.

CONFLICT OF INTEREST

The authors declare no conflict of interest.

References

1 

Masoli M, Fabian D, Holt S, Beasley R., Global Initiative for Asthma (GINA) Program. The global burden of asthma: executive summary of the GINA Dissemination Committee report. Allergy 2004; 59: 469-78.

2 

Castillo JR, Peters SP, Busse WW. Asthma exacerbations: pathogenesis, prevention, and treatment. J Allergy Clin Immunol Pract 2017; 5: 918-27.

3 

Wenzel SE. Asthma phenotypes: the evolution from clinical to molecular approaches. Nat Med 2012; 18: 716-25.

4 

Porpodis K, Tsiouprou I, Apostolopoulos A, et al. Eosinophilic asthma, phenotypes-endotypes and current biomarkers of choice. J Pers Med 2022; 12: 1093.

5 

Akdis CA, Arkwright PD, Brüggen MC, et al. Type 2 immunity in the skin and lungs. Allergy 2020; 75: 1582-605.

6 

Sugita K, Steer CA, Martinez-Gonzalez I, et al. Type 2 innate lymphoid cells disrupt bronchial epithelial barrier integrity by targeting tight junctions through IL-13 in asthmatic patients. J Allergy Clin Immunol 2018; 141: 300-10.

7 

Esteban-Gorgojo I, Antolín-Amérigo D, Domínguez-Ortega J, Quirce S. Non-eosinophilic asthma: current perspectives. J Asthma Allergy 2018; 11: 267-81.

8 

Komai T, Inoue M, Okamura T, et al. Transforming growth factor-β and interleukin-8 synergistically regulate humoral immunity via modulating metabolic signals. Front Immunol 2018; 9: 1364.

9 

Wen T, Rothenberg ME. The regulatory function of eosinophils. Microbiol Spectr 2016; 4: 10.1128/microbiolspec.MCHD-0020-2015.

10 

Carr TF, Kraft M. Use of biomarkers to identify phenotypes and endotypes of severe asthma. Ann Allergy Asthma Immunol 2018; 121: 414-20.

11 

Yancey SW, Keene ON, Albers FC, et al. Biomarkers of severe eosinophilic asthma. J Allergy Clin Immunol 2017; 140: 1509-18.

12 

Bochner BS. Novel therapies for eosinophilic disorders. Immunol Allergy Clin North Am 2015; 35: 577-98.

13 

O’Donnell MC, Ackerman SJ, Gleich GJ, Thomas LL. Activation of basophil and mast cell histamine release by eosinophil granule major basic protein. J Exp Med 1983; 157: 1981-91.

14 

Topic RZ, Dodig S. Eosinophil cationic protein–current concepts and controversies. Biochem Med 2011; 21: 111-21.

15 

McBrien CN, Menzies-Gow A. The biology of eosinophiles and their role in asthma. Front Med 2017; 4: 93.

16 

van Veen IH, ten Brinke A, Gauw SA, et al. Consistency of sputum eosinophilia in difficult-to-treat asthma: a 5-year follow-up study. J Allergy Clin Immunol 2009; 124: 615-7.

17 

de Groot JC, Storm H, Amelink M, et al. Clinical profile of patients with adult-onset eosinophilic asthma. ERJ Open Res 2016; 2: 00100-2015.

18 

Albinsson S, Lingblom C, Lundqvist C, et al. Eosinophils interact with thymocytes and proliferate in the human thymus. Eur J Immunol 2021; 51: 1539-41.

19 

Weller PF, Spencer LA. Functions of tissue-resident eosinophils. Nat Rev Immunol 2017; 17: 746-60.

20 

Lee EH, Itan M, Jang J, et al. Eosinophils support adipocyte maturation and promote glucose tolerance in obesity. Sci Rep 2018; 8: 9894.

21 

Kanda A, Yun Y, Bui DV, et al. The multiple functions and subpopulations of eosinophils in tissues under steady-state and pathological conditions. Allergol Int 2021; 70: 9-18.

22 

Mesnil C, Raulier S, Paulissen G, et al. Lung-resident eosinophils represent a distinct regulatory eosinophil subset. J Clin Invest 2016; 126: 3279-95.

23 

Benarafa C, Priebe GP, Remold-O’Donnell E. The neutrophil serine protease inhibitor serpinb1 preserves lung defense functions in Pseudomonas aeruginosa infection. J Exp Med 2007; 204: 1901-9.

24 

Ng FS, Wong KY, Guan SP, et al. Annexin-1-deficient mice exhibit spontaneous airway hyperresponsiveness and exacerbated allergen-specific antibody responses in a mouse model of asthma. Clin Exp Allergy 2011; 41: 1793-803.

25 

Lombardi C, Berti A, Cottini M. The emerging roles of eosinophils: implications for the targeted treatment of eosinophilic-associated inflammatory conditions. Curr Res Immunol 2022; 3: 42.

26 

Park YM, Bochner BS. Eosinophil survival and apoptosis in health and disease. Allergy Asthma Immunol Res 2010; 2: 87-100.

27 

Spector SL, Tan RA. Is a single blood eosinophil count a reliable marker for “eosinophilic asthma? J Asthma 2012; 49: 807-10.

28 

Price DB, Buhl R, Chan A, et al. Fractional exhaled nitric oxide as a predictor of response to inhaled corticosteroids in patients with non-specific respiratory symptoms and insignificant bronchodilator reversibility: a randomised controlled trial. Lancet Respir Med 2018; 6: 29-39.

29 

Zeiger RS, Schatz M, Zhang F, et al. Elevated exhaled nitric oxide is a clinical indicator of future uncontrolled asthma in asthmatic patients on inhaled corticosteroids. J Allergy Clin Immunol 2011; 128: 412-4.

30 

Matsumoto H. Roles of periostin in asthma. Adv Exp Med Biol 2019; 1132: 145-59.

31 

Izuhara K, Ohta S, Ono J. Using periostin as a biomarker in the treatment of asthma. Allergy Asthma Immunol Res 2016; 8: 491-8.

32 

Corren J, Lemanske RF, Hanania NA, et al. Lebrikizumab treatment in adults with asthma. N Engl J Med 2011; 365: 1088-98.

33 

Kanemitsu Y, Matsumoto H, Izuhara K, et al. Increased periostin associates with greater airflow limitation in patients receiving inhaled corticosteroids. J Allergy Clin Immunol 2013; 132: 305-12.

34 

Van Hulst G, Batugedara HM, Jorssen J, et al. Eosinophil diversity in asthma. Biochem Pharmacol 2020; 179: 113963.

35 

Conesa A, Tassinari P, Rivera H, et al. Hypodense eosinophils: characterization of surface molecule expression. Allergy Asthma Proc 2002; 23: 117-24.

36 

Prin L, Capron M, Tonnel AB, et al. Heterogeneity of human peripheral blood eosinophils: variability in cell density and cytotoxic ability in relation to the level and the origin of hypereosinophilia. Int Arch Allergy Appl Immunol 1983; 72: 336-46.

37 

Kuo HP, Yu TR, Yu CT. Hypodense eosinophil number relates to clinical severity, airway hyperresponsiveness and response to inhaled corticosteroids in asthmatic subjects. Eur Respir J 1994; 7: 1452-9.

38 

Evans PM, O’Connor BJ, Fuller RW, et al. Effect of inhaled corticosteroids on peripheral blood eosinophil counts and density profiles in asthma. J Allergy Clin Immunol 1993; 91: 643-50.

39 

Constantine GM, Klion AD. Recent advances in understanding the role of eosinophils. Fac Rev 2022; 11: 26.

40 

Dagher R, Kumar V, Copenhaver AM, et al. Novel mechanisms of action contributing to benralizumab’s potent anti-eosinophilic activity. Eur Respir J 2022; 59: 2004306,

41 

Harb H, Chatila TA. Mechanisms of dupilumab. Clin Exp Allergy 2020; 50: 5-14.

42 

Hamilton JD, Harel S, Swanson BN, et al. Dupilumab suppresses type 2 inflammatory biomarkers across multiple atopic, allergic diseases. Clin Exp Allergy 2021; 51: 915-31.

43 

Loureiro CC, Amaral L, Ferreira JA, et al. Omalizumab for severe asthma: beyond allergic asthma. Biomed Res Int 2018; 2018: 3254094.

44 

Beck LA, Marcotte GV, MacGlashan D, et al. Omalizumab-induced reductions in mast cell Fce psilon RI expression and function. J Allergy Clin Immunol 2004; 114: 527-30.

45 

Soumelis V, Reche PA, Kanzler H, et al. Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol 2002; 3: 673-80.

46 

Menzies-Gow A, Colice G, Griffiths JM, et al. NAVIGATOR: a phase 3 multicentre, randomized, double-blind, placebo-controlled, parallel-group trial to evaluate the efficacy and safety of tezepelumab in adults and adolescents with severe, uncontrolled asthma. Respir Res 2020; 21: 266.

47 

Porsbjerg CM, Sverrild A, Lloyd CM, et al. Anti-alarmins in asthma: targeting the airway epithelium with next-generation biologics. Eur Respir J 2020; 56: 2000260.

48 

Corren J, Menzies-Gow A, Chupp G, et al. Efficacy of tezepelumab in severe, uncontrolled asthma: pooled analysis of the PATHWAY and NAVIGATOR clinical trials. Am J Respir Crit Care Med 2023; 208: 13-24.

49 

Viswanathan RK, Busse WW. How to compare the efficacy of biologic agents in asthma. Ann Allergy Asthma Immunol 2020; 125: 137-49.

50 

Akenroye A, Lassiter G, Jackson JW, et al. Comparative efficacy of mepolizumab, benralizumab, and dupilumab in eosinophilic asthma: a Bayesian network meta-analysis. J Allergy Clin Immunol 2022; 150: 1097-105.e12.

51 

Edris A, De Feyter S, Maes T, et al. Monoclonal antibodies in type 2 asthma: a systematic review and network meta-analysis. Respir Res 2019; 20: 179.

52 

Ortega HG, Liu MC, Pavord ID, et al. Mepolizumab treatment in patients with severe eosinophilic asthma. N Engl J Med 2014; 371: 1198-207.

53 

Bleecker ER, FitzGerald JM, Chanez P, et al. Efficacy and safety of benralizumab for patients with severe asthma uncontrolled with high-dosage inhaled corticosteroids and long-acting β2-agonists (SIROCCO): a randomised, multicentre, placebo-controlled phase 3 trial. Lancet 2016; 388: 2115-27.

54 

Wang J, Slungaard A. Role of eosinophil peroxidase in host defense and disease pathology. Arch Biochem Biophys 2006; 445: 256-60.

55 

Mukherjee M, Bulir DC, Radford K, et al. Sputum autoantibodies in patients with severe eosinophilic asthma. J Allergy Clin Immunol 2018; 141: 1269-79.

56 

Carr TF, Berdnikovs S, Simon HU, et al. Eosinophilic bioactivities in severe asthma. World Allergy Organ J 2016; 9:21.

57 

Nakagome K, Nagata M. Involvement and possible role of eosinophils in asthma exacerbation. Front Immunol 2018; 9: 2220.

58 

Nakagome K, Nagata M. Pathogenesis of airway inflammation in bronchial asthma. Auris Nasus Larynx 2011; 38: 555-63.

59 

Barthel SR, Jarjour NN, Mosher DF, Johansson MW. Dissection of the hyperadhesive phenotype of airway eosinophils in asthma. Am J Respir Cell Mol Biol 2006; 35: 378-86.

60 

Kay AB, Phipps S, Robinson DS. A role for eosinophils in airway remodelling in asthma. Trends Immunol 2004; 25: 477-82.

61 

Flood-Page PT, Menzies-Gow AN, Kay AB, Robinson DS. Eosinophil’s role remains uncertain as anti-interleukin-5 only partially depletes numbers in asthmatic airway. Am J Respir Crit Care Med 2003; 167: 199-204.

62 

Wechsler ME, Klion AD, Paggiaro P, et al. Effect of dupilumab on blood eosinophil counts in patients with asthma, chronic rhinosinusitis with nasal polyps, atopic dermatitis, or eosinophilic esophagitis. J Allergy Clin Immunol Pract 2022; 10: 2695-709.

63 

Fulkerson PC, Fischetti CA, McBride ML, et al. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc Natl Acad Sci USA 2006; 103: 16418-23.

64 

Palframan RT, Collins PD, Williams TJ, Rankin SM. Eotaxin induces a rapid release of eosinophils and their progenitors from the bone marrow. Blood 1998; 91: 2240-8.

65 

Erin EM, Williams TJ, Barnes PJ, Hansel TT. Eotaxin receptor (CCR3) antagonism in asthma and allergic disease. Curr Drug Targets Inflamm Allergy 2002; 1: 201-14.

66 

Bartemes KR, Iijima K, Kobayashi T, et al. IL-33-responsive lineage-CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs. J Immunol 2012; 188: 1503-13.

67 

O’Sullivan JA, Chang AT, Youngblood BA, Bochner BS. Eosinophil and mast cell siglecs: from biology to drug target. J Leukoc Biol 2020; 108: 73-81.

68 

Kikly KK, Bochner BS, Freeman SD, et al. Identification of SAF-2, a novel siglec expressed on eosinophils, mast cells, and basophils. J Allergy Clin Immunol 2000; 105: 1093-100.

69 

Youngblood BA, Brock EC, Leung J, et al. AK002, a humanized sialic acid-binding immunoglobulin-like lectin-8 antibody that induces antibody-dependent cell-mediated cytotoxicity against human eosinophils and inhibits mast cell-mediated anaphylaxis in mice. Int Arch Allergy Immunol 2019; 180: 91-102.

70 

Bao L, Zhang H, Chan LS. The involvement of the JAK-STAT signaling pathway in chronic inflammatory skin disease atopic dermatitis. JAKSTAT 2013; 2: e24137.

71 

Luschnig P, Kienzl M, Roula D, et al. The JAK1/2 inhibitor baricitinib suppresses eosinophil effector function and restricts allergen-induced airway eosinophilia. Biochem Pharmacol 2021; 192: 114690.

72 

Vernet R, Matran R, Zerimech F, et al. Identification of novel genes influencing eosinophil-specific protein levels in asthma families. J Allergy Clin Immunol 2022; 150: 1168-77.

73 

Hammad H, Lambrecht BN. The basic immunology of asthma. Cells 2021; 184: 1469-85.

74 

Panch SR, Bozik ME, Brown T, et al. Dexpramipexole as an oral steroid-sparing agent in hypereosinophilic syndromes. Blood 2018; 132: 501-9.

75 

Siddiqui S, Wenzel SE, Bozik ME, et al. Safety and Efficacy of Dexpramipexole in Eosinophilic Asthma (EXHALE): a randomized controlled trial. J Allergy Clin Immunol 2023; 152: 1121-30.

76 

Lambrecht BN, Hammad H. The immunology of asthma. Nat Immunol 2015; 16: 45-56.

77 

Leckie MJ, ten Brinke A, Khan J, et al. Effects of an interleukin-5 blocking monoclonal antibody on eosinophils, airway hyper-responsiveness, and the late asthmatic response. Lancet 2000; 356: 2144-8.

78 

Moran AM, Ramakrishnan S, Borg CA, et al. Blood eosinophil depletion with mepolizumab, benralizumab, and prednisolone in eosinophilic asthma. Am J Respir Crit Care Med 2020; 202: 1314-6.

79 

Kolbeck R, Kozhich A, Koike M, et al. MEDI-563, a humanized anti-IL-5 receptor alpha mAb with enhanced antibody-dependent cell-mediated cytotoxicity function. J Allergy Clin Immunol 2010; 125: 1344-53.

80 

Johansson MW, Gunderson KA, Kelly EA, et al. Anti-IL-5 attenuates activation and surface density of β(2)-integrins on circulating eosinophils after segmental antigen challenge. Clin Exp Allergy 2013; 43: 292-301.

81 

Johansson MW. Eosinophil activation status in separate compartments and association with asthma. Front Med (Lausanne) 2017; 4: 75.

82 

Novosad J, Krčmová I, Souček O, et al. Subsets of eosinophils in asthma, a challenge for precise treatment. Int J Mol Sci 2023; 24: 5716.

Copyright: © Polish Society of Allergology This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivatives 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
 
Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.