eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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1/2014
vol. 95
 
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abstract:
Review paper

Application of epoxy functional silanes in the preparation of DNA microarrays

Emilia Frydrych-Tomczak
,
Barbara Uszczyńska
,
Tomasz Ratajczak
,
Wojciech T. Markiewicz
,
Marek Figlerowicz
,
Marek Nowicki
,
Hieronim Maciejewski
,
Marcin K. Chmielewski

BioTechnologia vol. 95(1) C pp. 5-16 C 2014
Online publish date: 2014/10/23
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Nucleic acid microarrays have recently become one of the basic techniques in the study of gene expression.

Owing to progress in the field of miniaturization, thousands of oligonucleotides differing in terms of their sequences

can be systematically placed on a small area of a solid support (usually glass). These probes are capable of

simultaneously interacting with a large number of longer nucleic acids from particular genes. Nucleic acid microarray

construction technology consists, primarily, of the appropriate functionalization of a glass surface with the

use of organofunctional silanes. Oligonucleotides known as probes are attached to a functionalized surface using,

for instance, the lithography technique and, after being linked to the surface, they are subjected to hybridization

with complementary and labelled fragments of nucleic acids known as samples of unknown sequences. In this

paper, we present a method for constructing DNA microarrays that is based on the use of microscopic slides modified

with epoxy functional group-containing silanes. This study was aimed at optimizing the production of DNA

microarrays. The study tested the usefulness of four different epoxy functional silanes with one or three alkoxy

groups. In addition, slides were silanized with the use of alkylsilane. The glass slides were characterized using

a goniometer and an atomic force microscope (AFM). The synthesized amino linker-containing oligonucleotide

probes were printed onto the glass slides in order to check the effectiveness of their attachment to the solid

surface.
keywords:

DNA microarrays, organofunctional silanes, glass supports, oligonucleotides



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