eISSN: 2299-551X
ISSN: 0011-4553
Journal of Stomatology
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1/2017
vol. 70
 
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abstract:

Antifungal properties of selected calcium silicate-based cements

Joanna Możyńska
1
,
Alicja Nowicka
2
,
Magdalena Kaczała
3
,
Mariusz Lipski
4
,
Marcin Metlerski
1
,
Agnieszka Gerula-Szymańska
1
,
Jadwiga Buczkowska-Radlińska
5

  1. Studium Doktoranckie Wydziału Lekarsko-Stomatologicznego, Pomorski Uniwersytet Medyczny w Szczecinie, Polska Doctoral Studies of the Faculty of Dentistry, Pomeranian Medical University in Szczecin, Poland Head: dr hab. D. Lichota, prof. PUM
  2. Collegium Medicum UMK
  3. Katedra Mikrobiologii, Immunologii i Medycyny Laboratoryjnej, Pomorski Uniwersytet Medyczny w Szczecinie, Polska Department of Microbiology and Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Poland Head: prof. B. Dołęgowska
  4. Zakład Stomatologii Zachowawczej Przedklinicznej i Endodoncji Przedklinicznej Pomorskiego Uniwersytetu Medycznego w Szczecinie
  5. Z Zakładu Stomatologii Zachowawczej Pomorskiego Uniwersytetu Medycznego w Szczecinie
Online publish date: 2017/04/23
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Introduction. The role of fungi in the failure of root
canal treatment has been well documented, pointing
to Candida albicans as the most common species.
Consequently, the material forming the protective
barrier between periodontal tissues and the root canal
system should demonstrate sufficient bactericidal
and fungicidal activity. Aim of the study. To compare
antifungal activity against C. albicans of ProRoot
MTA, MTA Plus, Biodentine and MTA+. Material and
methods. The cements were prepared in accordance
with the manufacturer’s instructions, packed into
sections of sterile catheters, and immersed for three
hours in sterile water. They were next transferred to
48-well polystyrene plates and incubated at 37°C with
106 cells/ml of C. albicans. Samples were immersed in
phosphate-buffered saline (PBS) for 24h or 48h and
plated on Sabouraud medium. The number of colonies
of C. albicans was determined after 48h. At the same
time, the pH of the solution was measured after 3h,24h, and 48h of incubation. Results. Fungicidal
activity in decreasing strength was as follows: MTA
Plus, ProRoot MTA, Biodentine, and MTA+. MTA
Plus had the highest pH of the incubation solution.
Conclusions. Considering the antifungal effect,
all evaluated biomaterials are recommendable in
endodontic treatment.

 
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